Review



secondary antibody incubation  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher secondary antibody incubation
    Secondary Antibody Incubation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/secondary+antibody+incubation/SUCROSE+EP%2FBP%2FNF+12KG/pm41977223-238-0-6
    Average 99 stars, based on 1 article reviews
    secondary antibody incubation - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Implications of neonatal absence of innate immune mediated NFκB/AP1 signaling in the murine liver.
    Article Snippet: BACKGROUND: The developmental immaturity of the innate immune system helps explains the increased risk of infection in the neonatal period.. Importantly, innate immune signaling pathways such as p65/NFκB and c-Jun/AP1 are responsible for the prevention of hepatocyte apoptosis in adult animals, yet whether developmental immaturity of these pathways increases the risk of hepatic injury in the neonatal period is unknown.. METHODS: Using a murine model of endotoxemia (LPS 5mg/kg IP x 1) in neonatal (P3) and adult mice, we evaluated histologic evidence of hepatic injury and apoptosis, presence of p65/NFκB and c-Jun/AP1 activation and associated transcriptional regulation of apoptotic genes.

    Article Title: Prenatal liver is resistant to low glucose-induced inhibition of mTORC1
    Article Snippet: .. The dilution ratios were LAMP2 (1:120, 120 μl diluent +1 μl antibody), mTOR (1:80, 80 μl diluent +1 μl antibody). (8) Secondary antibody incubation: After washing with PBS, incubated the cells with Alexa Fluor 488- or 594-conjugated secondary antibodies (Invitrogen, Carlsbad, CA, USA) for overnight at 4°C, protected from light. ..

    Article Title: Radiotherapy-Induced Neurocognitive Impairment Is Driven by Heightened Apoptotic Priming in Early Life and Prevented by Blocking BAX
    Article Snippet: Antibodies: cleaved caspase-3 (Cell Signaling Technology #9661, RRID:AB_2341188), SOX2 (Cell Signaling Technology #4900, RRID:AB_10560516), and NG2 (Abcam ab273443, RRID:AB_2940856). .. Secondary antibody incubation at 1:200 dilution (Thermo Fisher Scientific A-11008, RRID:AB_143165) and DAPI staining (Abcam ab228549) was performed the next day, with cover slipping using vector labs antifade mounting media (Vector Laboratories H-1000-10). .. Slides were imaged on a Zeiss Axio Observer and quantified using CellProfiler ( ).

    Article Title: A population of Vasa2 and Piwi1 expressing cells generates germ cells and neurons in a sea anemone
    Article Snippet: .. Hoechst or DAPI nuclear staining (ThermoFisher) and secondary antibody incubation was performed in 1x PBS/5% NGS/0.2% Triton X-100 overnight at 4 °C using the following antibodies: goat-anti-mouse-Alexa488/568 (LifeTech A11001, A11004), goat-anti-rabbit-DyLight488 (LifeTech 35552), goat-anti-rabbit-Alexa568/647 (LifeTech A11011, A21244) and goat-anti-rat-Alexa633 (LifeTech A21094). ..

    Article Title: Windows of susceptibility to neonatal acute kidney injury and nephron loss in a rabbit model.
    Article Snippet: Single molecule fluorescence in situ hybridization using RNAScope RNAScope was performed using the Multiplex Fluorescent V2 Assay (Advanced Cell Diagnostics, Inc.) according to the manufacturer’s protocol. .. After completion of the manufacturer’s recommendation for RNAScope V2 kit, slides were incubated in biotinylated LTL (Vector Labs) and Guinea pig anti- Krt8/18 (Progen) overnight at 4 °C, followed by wash and secondary antibody incubation for 1 h with streptavidin conjugated to Alexa FluorTM 647 (ThermoScientific) and Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 750)(Abcam). ..

    Article Title: BACE1 regulates sleep–wake cycle through both enzymatic and non–enzymatic actions
    Article Snippet: .. Sections were incubated with rabbit anti-synaptoporin (SynapticSystems, 102 002; 1:500) or rabbit anti-BACE (Cell Signaling, #5606; 1:250) antibodies at room temperature for 24 h. Secondary antibody incubation (donkey anti-rabbit Alexa Fluor 488, Molecular Probes, A-11029; 1:500) was performed at 4°C for 24 h. Sciatic nerve sections were processed similarly to the cochlear whole mounts, using the same blocking and antibody protocols. .. After immunostaining, slides were rinsed with PBS, mounted with DAPI-containing medium (Carl Roth), sealed, and stored at 4°C.

    Article Title: Naproxen and Ibuprofen Exposure Alter the Blood-Testis Barrier in a Novel In Vitro Model.
    Article Snippet: For primary antibodies, SOX9 and WT1 (Table S2 and Figure S5B), incubation was performed overnight at 4 ◦C in blocking buffer, followed by three washes in 1X PBS with 0.1% Triton X for 10 min each at room temperature (RT) on an LSE Orbital Shaker (Corning®). .. Secondary antibody incubation (1:1000, Alexa FluorTM Invitrogen, Carlsbad, CA, USA, Table S2) in blocking buffer occurred for 45 min at room temperature on the Orbital Shaker or overnight at 4 ◦C. .. Following the three washes described above, the samples were co-stained with Hoechst 33342 (1:1000, Cat. No. H1399, Invitrogen, Carlsbad, CA, USA).

    Article Title: Windows of susceptibility to neonatal acute kidney injury and nephron loss in a rabbit model
    Article Snippet: .. After completion of the manufacturer’s recommendation for RNAScope V2 kit, slides were incubated in biotinylated LTL (Vector Labs) and Guinea pig anti- Krt8/18 (Progen) overnight at 4 °C, followed by wash and secondary antibody incubation for 1 h with streptavidin conjugated to Alexa FluorTM 647 (ThermoScientific) and Goat Anti-Guinea pig IgG H&L (Alexa Fluor ® 750)(Abcam). ..

    Staining:

    Article Title: Radiotherapy-Induced Neurocognitive Impairment Is Driven by Heightened Apoptotic Priming in Early Life and Prevented by Blocking BAX
    Article Snippet: Antibodies: cleaved caspase-3 (Cell Signaling Technology #9661, RRID:AB_2341188), SOX2 (Cell Signaling Technology #4900, RRID:AB_10560516), and NG2 (Abcam ab273443, RRID:AB_2940856). .. Secondary antibody incubation at 1:200 dilution (Thermo Fisher Scientific A-11008, RRID:AB_143165) and DAPI staining (Abcam ab228549) was performed the next day, with cover slipping using vector labs antifade mounting media (Vector Laboratories H-1000-10). .. Slides were imaged on a Zeiss Axio Observer and quantified using CellProfiler ( ).

    Article Title: A population of Vasa2 and Piwi1 expressing cells generates germ cells and neurons in a sea anemone
    Article Snippet: .. Hoechst or DAPI nuclear staining (ThermoFisher) and secondary antibody incubation was performed in 1x PBS/5% NGS/0.2% Triton X-100 overnight at 4 °C using the following antibodies: goat-anti-mouse-Alexa488/568 (LifeTech A11001, A11004), goat-anti-rabbit-DyLight488 (LifeTech 35552), goat-anti-rabbit-Alexa568/647 (LifeTech A11011, A21244) and goat-anti-rat-Alexa633 (LifeTech A21094). ..

    Next-Generation Sequencing:

    Article Title: A population of Vasa2 and Piwi1 expressing cells generates germ cells and neurons in a sea anemone
    Article Snippet: .. Hoechst or DAPI nuclear staining (ThermoFisher) and secondary antibody incubation was performed in 1x PBS/5% NGS/0.2% Triton X-100 overnight at 4 °C using the following antibodies: goat-anti-mouse-Alexa488/568 (LifeTech A11001, A11004), goat-anti-rabbit-DyLight488 (LifeTech 35552), goat-anti-rabbit-Alexa568/647 (LifeTech A11011, A21244) and goat-anti-rat-Alexa633 (LifeTech A21094). ..

    RNAscope:

    Article Title: Windows of susceptibility to neonatal acute kidney injury and nephron loss in a rabbit model.
    Article Snippet: Single molecule fluorescence in situ hybridization using RNAScope RNAScope was performed using the Multiplex Fluorescent V2 Assay (Advanced Cell Diagnostics, Inc.) according to the manufacturer’s protocol. .. After completion of the manufacturer’s recommendation for RNAScope V2 kit, slides were incubated in biotinylated LTL (Vector Labs) and Guinea pig anti- Krt8/18 (Progen) overnight at 4 °C, followed by wash and secondary antibody incubation for 1 h with streptavidin conjugated to Alexa FluorTM 647 (ThermoScientific) and Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 750)(Abcam). ..

    Article Title: Windows of susceptibility to neonatal acute kidney injury and nephron loss in a rabbit model
    Article Snippet: .. After completion of the manufacturer’s recommendation for RNAScope V2 kit, slides were incubated in biotinylated LTL (Vector Labs) and Guinea pig anti- Krt8/18 (Progen) overnight at 4 °C, followed by wash and secondary antibody incubation for 1 h with streptavidin conjugated to Alexa FluorTM 647 (ThermoScientific) and Goat Anti-Guinea pig IgG H&L (Alexa Fluor ® 750)(Abcam). ..

    Blocking Assay:

    Article Title: BACE1 regulates sleep–wake cycle through both enzymatic and non–enzymatic actions
    Article Snippet: .. Sections were incubated with rabbit anti-synaptoporin (SynapticSystems, 102 002; 1:500) or rabbit anti-BACE (Cell Signaling, #5606; 1:250) antibodies at room temperature for 24 h. Secondary antibody incubation (donkey anti-rabbit Alexa Fluor 488, Molecular Probes, A-11029; 1:500) was performed at 4°C for 24 h. Sciatic nerve sections were processed similarly to the cochlear whole mounts, using the same blocking and antibody protocols. .. After immunostaining, slides were rinsed with PBS, mounted with DAPI-containing medium (Carl Roth), sealed, and stored at 4°C.

    Article Title: Naproxen and Ibuprofen Exposure Alter the Blood-Testis Barrier in a Novel In Vitro Model.
    Article Snippet: For primary antibodies, SOX9 and WT1 (Table S2 and Figure S5B), incubation was performed overnight at 4 ◦C in blocking buffer, followed by three washes in 1X PBS with 0.1% Triton X for 10 min each at room temperature (RT) on an LSE Orbital Shaker (Corning®). .. Secondary antibody incubation (1:1000, Alexa FluorTM Invitrogen, Carlsbad, CA, USA, Table S2) in blocking buffer occurred for 45 min at room temperature on the Orbital Shaker or overnight at 4 ◦C. .. Following the three washes described above, the samples were co-stained with Hoechst 33342 (1:1000, Cat. No. H1399, Invitrogen, Carlsbad, CA, USA).



    Similar Products

    94
    Sino Biological incubation goat anti human fc secondary antibody hrp
    Incubation Goat Anti Human Fc Secondary Antibody Hrp, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/secondary+antibody+incubation/Goat+Anti-Human+IgG-Fc+Secondary+Antibody/us12539336-787-39-46
    Average 94 stars, based on 1 article reviews
    incubation goat anti human fc secondary antibody hrp - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    99
    Thermo Fisher secondary antibody incubation
    Secondary Antibody Incubation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/secondary+antibody+incubation/SUCROSE+EP%2FBP%2FNF+12KG/pm41977223-238-0-6
    Average 99 stars, based on 1 article reviews
    secondary antibody incubation - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec secondary streptavidin microbeads incubation
    Secondary Streptavidin Microbeads Incubation, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/secondary+antibody+incubation/Streptavidin+MicroBeads/pm41860446-89-35-44
    Average 96 stars, based on 1 article reviews
    secondary streptavidin microbeads incubation - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc secondary antibody incubation
    Secondary Antibody Incubation, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/secondary+antibody+incubation/Anti-rabbit+IgG%2C+HRP-linked+Antibody/pmc12808835-428-0-7
    Average 99 stars, based on 1 article reviews
    secondary antibody incubation - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc incubation withhrp conjugated goat antirabbit secondary antibody
    Incubation Withhrp Conjugated Goat Antirabbit Secondary Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/secondary+antibody+incubation/Anti-rabbit+IgG%2C+HRP-linked+Antibody/pm41487047-121-16-22
    Average 99 stars, based on 1 article reviews
    incubation withhrp conjugated goat antirabbit secondary antibody - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    97
    Bio-Rad secondary antibody incubation
    Secondary Antibody Incubation, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/secondary+antibody+incubation/Secondary+Antibody+(Goat+Anti-Rabbit+Antibody+Conjugated+to+Horseradish+Peroxidase)/pm41385600-280-3-11
    Average 97 stars, based on 1 article reviews
    secondary antibody incubation - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    96
    Rockland Immunochemicals secondary antibody incubation
    Secondary Antibody Incubation, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/secondary+antibody+incubation/Anti-Rabbit+IgG/bio_rxiv__64898__2025__12__05__692364-254-9-21
    Average 96 stars, based on 1 article reviews
    secondary antibody incubation - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    86
    Huabio Inc secondary antibody incubation
    Secondary Antibody Incubation, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/secondary+antibody+incubation/antibody+incubation+primary/pm41135741-88-0-6
    Average 86 stars, based on 1 article reviews
    secondary antibody incubation - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    Image Search Results